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	Provedor de dados BJMBR (2) Autor Prado,V.F. (2) Beirão,P.S.L. (1) Cruz,J.S. (1) Guidine,P.A.M. (1) Mello,L.E. (1) Moraes,M.F.D. (1) Moreira,K.M. (1) Mudado,M.A. (1) Prado,M.A.M. (1) Queiroz,C.M. (1) Rezende,G.H.S. (1) Rodrigues,A.L. (1) Tiba,P.A. (1) Tufik,S. (1) Mais... Palavra-chave Acetylcholine (1) Calcium channel (1) Contextual fear conditioning (1) Electrophysiology (1) GH3 cells (1) Intermediate sleep (1) Memory (1) Neurodegenerative disorders (1) RT-PCR (1) Sleep-wake cycle (1) T-type (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última CaV 3.1 and CaV 3.3 account for T-type Ca2+ current in GH3 cells BJMBR Mudado,M.A.; Rodrigues,A.L.; Prado,V.F.; Beirão,P.S.L.; Cruz,J.S.. T-type Ca2+ channels are important for cell signaling by a variety of cells. We report here the electrophysiological and molecular characteristics of the whole-cell Ca2+ current in GH3 clonal pituitary cells. The current inactivation at 0 mV was described by a single exponential function with a time constant of 18.32 ± 1.87 ms (N = 16). The I-V relationship measured with Ca2+ as a charge carrier was shifted to the left when we applied a conditioning pre-pulse of up to -120 mV, indicating that a low voltage-activated current may be present in GH3 cells. Transient currents were first activated at -50 mV and peaked around -20 mV. The half-maximal voltage activation and the slope factors for the two conditions are -35.02 ± 2.4 and 6.7 ± 0.3 mV (pre-pulse of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium channel; T-type; Electrophysiology; RT-PCR; GH3 cells. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600020 Sleep pattern and learning in knockdown mice with reduced cholinergic neurotransmission BJMBR Queiroz,C.M.; Tiba,P.A.; Moreira,K.M.; Guidine,P.A.M.; Rezende,G.H.S.; Moraes,M.F.D.; Prado,M.A.M.; Prado,V.F.; Tufik,S.; Mello,L.E.. Impaired cholinergic neurotransmission can affect memory formation and influence sleep-wake cycles (SWC). In the present study, we describe the SWC in mice with a deficient vesicular acetylcholine transporter (VAChT) system, previously characterized as presenting reduced acetylcholine release and cognitive and behavioral dysfunctions. Continuous, chronic ECoG and EMG recordings were used to evaluate the SWC pattern during light and dark phases in VAChT knockdown heterozygous (VAChT-KDHET, n=7) and wild-type (WT, n=7) mice. SWC were evaluated for sleep efficiency, total amount and mean duration of slow-wave, intermediate and paradoxical sleep, as well as the number of awakenings from sleep. After recording SWC, contextual fear-conditioning tests were used... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sleep-wake cycle; Intermediate sleep; Acetylcholine; Contextual fear conditioning; Memory; Neurodegenerative disorders. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013001000844 Registros recuperados: 2 Primeira ... 1 ... Última

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